Painted in 1492, this work by Carlo Crivelli may be the earliest dated picture of the Virgin of the Immaculate Conception. The symbols derive from the Bible, including the Book of Revelation and The Song of Songs. Here, the Virgin's purity is symbolised by a lily in a pure crystal glass. The painting comes from the Franciscan church of San Francesco, Pergola, central Italy. It hangs in Room 59: http://bit.ly/2haE6ID
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